 Wysłany: Czw 8:38, 02 Gru 2010 Temat postu: ugg boots australia Ethylene signal transcription |
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| Ethylene signal transcription factor LeERF2 in E. coli expression and purification
gpathway: newinsigh ~. CurrentOpinion / nP / antB / o / ogy, 2O04 ,ugg boots australia,7:40-49 [3] HaoDY, Ohme-Takagi. UniquemodeofGCCboxrecognitionbytheDNA-bindingdomainofethyleneresponsiveelement-bindingfactor (ERFdomain) inplants. JB / o / Chem, 1998,sheepskin ugg boots,273:26857.26861 [4] ParkJM, ParkCJ,MAC Cosmetics Cheap, leeSB, eta1. OverexpressionoftheTo-baccoTsilgeneencodingallEREBP/AP2-typetranscriptionfactorenhancesresistancengai ~ tpathogenattackandosmoticgssintobacco. ZePt ~ mt, 2 (I, 13:1035.1046 ExpressionandpurificationoftomatoethylenesignaltranscriptionfactorLeERF2inE.coilHongxing,MAC Wholesale, ZhuBenzhong, XieYuanhong \-quencingresultindicatedthattheidentitybetweentheclonedgeneandthepublishedgenewas100% .ThePCRproductwassubclonedintotheprokaryoticproteinexpressionvectorpET-30atogeneraterecombinantplasmidpET-LeERF2inE.coli21.LeERF2proteinWasexpressedunder1.Ommol/LWIGinductionfor3hat37 ℃. TheexpressionlevelofLeERF2Wasabout58% oftotalcellularproteins.ThepurificationlevelofLeERF2purifiedbyNi-NTAagarosecolumnWasabout99%. ThesilverstainingelectrophoreticmobilityshiftassayshowedthatLeERF2proteincouldbindtothepromoteroftheTLBP1genecodingforpathogenesis-relatedchitinasewithGCCbox.Keywords: tomato, ethylene, LeERF2,PUMA trionfo baylee shoes, pmkaryoticproteinexpression, electrophoreticmobilityshiftassay (EM-sA) ・ - --- 550 ・---- 相关的主题文章： sheepskin ugg boots 第七届海内外青年设计与制造科学 MAC 120 color eyeshadow Freak disease _823 PUMA BAYLEE FUTURE CAT Hydrothermal Synthesis of N |
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